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Original Research Article | OPEN ACCESS

Assessment of activity and mechanism of action of β-D-glucan against dengue virus

Yonghong Song1, Wenzhi Zhang2, Ravindran Jaganathan3

1Department of Anatomy, Medical College, Hebei University of Engineering, Congtai Street 83, Handan 056002, Hebei Province, China; 2Innoscience Research Sdn Bhd, Suites B-5-7, Level 5, Sky Park @ One City, Jalan USJ 25/1, 47650 Subang Jaya, Selangor; 3Pathology Unit, Lab Based Dept., Faculty of Medicine, Universiti Kuala Lumpur-Royal College of Medicine Perak (UniKL-RCMP), No. 3 Jalan Greentown, Ipoh-30450, Perak, Malaysia.

For correspondence:-  Ravindran Jaganathan   Email: jravimicro@gmail.com   Tel:+60176184663

Accepted: 14 May 2018        Published: 30 June 2018

Citation: Song Y, Zhang W, Jaganathan R. Assessment of activity and mechanism of action of β-D-glucan against dengue virus. Trop J Pharm Res 2018; 17(6):1061-1066 doi: 10.4314/tjpr.v17i6.12

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To assess the antiviral efficiency of β-D-glucan (BDG) on human liver cell line (WRL68) infected with dengue virus (DENV).
Methods: Cytotoxic activity was assessed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Solid-phase virus binding assay was used to determine the presence of a chemical affinity between dengue virus type 2 (DENV-2) and BDG. Plaque formation assay was performed to measure the suppression of DENV-2.
Results: Plaque formation assay results revealed that the inhibition of DENV infection by BDG was effective at 400 μg/mL which occurred by inhibiting virus replication. BDG inhibited DENV replication and produced minimal toxicity on WRL68 cells at 600 µg/mL in a concentration-dependent manner. Treatment of DENV-2 with the highest concentration of the BDG resulted in 60, 55, and 50 % viability at 24, 48, and 72 h, respectively. Plaque formation and binding efficiency data confirmed that BDG protected the WRL68 cells against DENV-2.
Conclusion: The results indicate that in infected cells, β-D-glucan was found to be potent in inhibiting replication of the dengue virus.

Keywords: Dengue, ^6;-D-glucan, Polysaccharide, Antiviral, Plaque formation, Binding efficiency

Impact Factor
Thompson Reuters (ISI): 0.523 (2021)
H-5 index (Google Scholar): 39 (2021)

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